Analytical methodologies to assure successful peptide synthesis when utilizing polystyrene/DVB resins

Solid-phase peptide synthesis has become an important technique as the method of choice to deliver high quality peptide entities at both laboratory and production scale. The polymeric support (i.e. the resin) is the most essential part of the process as it covalently holds the growing peptide through the various synthetic steps. Several key features of the resin must be addressed before initiation of the synthesis to ensure successful generation of the desired peptide. In this paper, several PuroSynthTM peptide synthesis resins were examined for a range of aspects including morphological characteristics, mechanical stability and distribution of functional groups. Additionally, to demonstrate the effect of these aspects, the Purosynth™ CTC resin was used to synthesise a heptapeptide analogue to the antibiotic darobactin with >95 % purity.